Jump Start into Targeted Metabolomics

Highlights:

  • Broad metabolic and lipid profiling on Thermo ScientificTM Q ExactiveTM mass spectrometers:
    The AbsoluteIDQ® p400 HR Kit provides quantitative analysis of up to 408 metabolites, including 365 lipids.
  • Excellent reproducibility:
    The kit ensures batch-to-batch and inter-laboratory comparability.
  • Ready-to-use:
    No method development required, method is established in your laboratory within one day.
  • High throughput:
    One kit analyzes up to 82 samples within 38 hours.
  • Small sample volume:
    Just 10 µL of biofluid needed (various matrices have been tested).

Metabolomics is a powerful technology that can be universally applied in biomedical research. Despite the fact that there is no single analytical technique that can cover the whole metabolome, standardized and reproducible quantification of a wide range of metabolites in your samples can be an important step towards a successful application of Metabolomics to your research project.

p400 HR pic frontpage 100dpi

The AbsoluteIDQ® p400 HR Kit represents the first standardized kit solution for Targeted Metabolomics on high resolution, accurate mass (HRAM) Q ExactiveTM mass spectrometers. It combines broad analyte coverage, the main advantage of metabolic profiling (i.e. hypothesis generating approaches), with quantitative, highly reproducible results, the main advantage of targeted metabolomics (i.e. hypothesis driven approaches).

From a tiny amount of sample, you can analyze up to 408 metabolites in your own laboratory or in a collaborating laboratory of your choice. The AbsoluteIDQ® p400 HR Kit covers up to 408 compounds from 11 metabolite classes, including core metabolic pathways as well as a large range of lipid metabolites: 

-   

Small Molecules     Polar Lipids Neutral Lipids
Amino Acids Phosphatidylcholines Acylcarnitines
Biogenic Amines Lysophosphatidylcholines Diglycerides
Hexoses Sphingomyelins Triglycerides
  Ceramides Cholesteryl Esters
     

 

The AbsoluteIDQ® p400 HR Kit has been developed for the following UHPLC-MS platforms:
- Q ExactiveTM
- Q ExactiveTM Focus
- Q ExactiveTM Plus
- Q ExactiveTM HF

List of Metabolites

Download the detailed kit information and contact us for further information!

View Sample Preparation Guidelines

For Research Use Only. Not for use in diagnostic procedures.