AbsoluteIDQ® Bile Acids kit

Bile-Acids

AbsoluteIDQ® Bile acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ bile acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease. The Biocrates® Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).
biocrates AbsoluteIDQ Bile Acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Ready-to-use_Kitbox

All in one box

  • Includes all necessary reagents and methods
Functional-Microbiomics_Mikroben

High throughput

  • Up to 400 samples per instrument per week
Ready-to-use_Kitbox

Proven reproducibility

Superior-Data-Quality_Zielscheibe

Superior data quality

  • Outstanding accuracy and precision of results

Kit components

  • Patented 96-well filter plates with pre-set internal standards
  • Calibration standards and quality controls
  • Test samples for system suitability test
  • Quick-start guide and user manual
  • Sample preparation protocol
  • Instrument-specific methods

Kit requirements

  • Cloud-based software (on-premise option also available)
  • Complete experimental process guidance
  • Automated quantification, technical validation and report generation
  • MetaboINDICATOR™ with biologically relevant metabolite sums and ratios
  • Compatible with Agilent 6495 C; SCIEX 6500+, 6500, 5500+, 5500, 4500, 4000; Waters TQ-S, TQ-S micro; Thermo TSQ Vantage
  • Validated LC column
  • Compatible with ultra high performance liquid chromatography (UHPLC)

Metabolite coverage

  • 10 primary bile acids: produced by the liver (CA, GCA, TCA, CDCA, GCDCA, TCDCA, α-MCA, β-MCA, ω-MCA, TMCA(α+β))
  • 10 secondary bile acids: produced by gut bacteria from primary bile acids (DCA, GDCA, TDCA, LCA, GLCA, TLCA, UDCA, GUDCA, TUDCA, HDCA)

MetaboINDICATOR™

Translate metabolomics into knowledge with one click

Calculating and understanding metabolite sums and ratios makes your biological data interpretation easier and more comprehensive. That is why we created  MetaboINDICATOR™ as an add-on tool to WebIDQ. With one click you can find out how metabolite sums and ratios are associated with related biological functions so your data has meaningful impact.

MetaboINDICATOR™ is more than an additional dimension to your data analysis; it unravels the scientific context behind your data. All in one click.

Related assets

Absolute IDQ Bile Acids

brochure

AbsoluteIDQ® Bile Acids kit
pdf ~ 1 MB
download

biocrates kit technology

brochure

biocrates kit technology
pdf ~ 6 MB
download

Services

brochure

biocrates services
pdf ~ 1.5 MB
download

WebIDQ software

brochure

WebIDQ software
pdf ~ 2 MB
download

Talk to an expert

biocrates life sciences ag
Eduard-Bodem-Gasse 8
6020 Innsbruck | AUSTRIA
Phone +43 512 579823
Fax +43 512 579823-329

biocrates inc.
26895 Aliso Creek Rd Ste B-426
Aliso Viejo, CA 92656-5301
Phone +1 774 424 8150
Operating hours: Mo – Fr | 9 am – 7 pm (EST)